The GTX60902 reacts to Histone H3 acetylated at Lysine 56(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
![ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179]. Red : Primary antibody Green : Actin](https://www.genetex.com/upload/website/prouct_img/normal/GTX60902/GTX60902_20200909_ICCIF_170_w_23061123_144.webp "ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179]. Red : Primary antibody Green : Actin")
![ICC/IF analysis of HepG2 cells using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60902/GTX60902_20200909_ICCIF_194_w_23061123_867.webp "ICC/IF analysis of HepG2 cells using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].")
![WB analysis of extracts of HeLa cells treated (Lane 1) with sodium butyrate and recombinant Histone H3.3 protein (Lane 2) using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60902/GTX60902_20200909_WB_245_w_23061123_610.webp "WB analysis of extracts of HeLa cells treated (Lane 1) with sodium butyrate and recombinant Histone H3.3 protein (Lane 2) using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].")
The GTX60902 reacts to Histone H3 acetylated at Lysine 56(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Histone H3K56ac (Acetyl Lys56) antibody [RM179]
GTX60902
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product NameHistone H3K56ac (Acetyl Lys56) antibody [RM179]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.
- Application Supplier NoteWB: 1 microg/mL - 2 microg/mL. ICC/IF: 0.5 microg/mL - 2 microg/mL. ELISA: 0.5 microg/mL - 1 microg/mL. IHC: 1 microg/mL - 10 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM179
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Human CRL4DDB2 ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1. Zhu Q et al., 2017 Nov 28, OncotargetRead more