ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (2 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).