The GTX60885 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetyl-ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
![ChIP analysis of HeLa cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX60885/GTX60885_20200909_ChIPassay_6_w_23061123_861.webp "ChIP analysis of HeLa cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg")
![Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60885/GTX60885_20200909_ELISA_129_w_23061123_253.webp "Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.")
![Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60885/GTX60885_20200909_ELISA_138_w_23061123_232.webp "Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.")
![ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Red : Primary antibody Green : Actin](https://www.genetex.com/upload/website/prouct_img/normal/GTX60885/GTX60885_20200909_ICCIF_172_w_23061123_262.webp "ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Red : Primary antibody Green : Actin")
![ICC/IF analysis of HepG2 cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60885/GTX60885_20200909_ICCIF_195_w_23061123_925.webp "ICC/IF analysis of HepG2 cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].")
![WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Dilution : 0.25microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60885/GTX60885_20200909_WB_264_w_23061123_713.webp "WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Dilution : 0.25microg/ml")
The GTX60885 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetyl-ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Histone H3K9ac (Acetyl Lys9) antibody [RM161] - Orthogonal Validated
GTX60885
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetH3C1
Overview
- SupplierGeneTex
- Product NameHistone H3K9ac (Acetyl Lys9) antibody [RM161] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM161
- ConjugateUnconjugated
- Gene ID8350
- Target nameH3C1
- Target descriptionH3 clustered histone 1
- Target synonymsH3 histone family, member A; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3.1; histone H3/a
- HostRabbit
- IsotypeIgG
- Modification TypeAcetylated
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203