ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (2 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).