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Western Blot analysis of Human normal liver tissue and hepg2 cell, Jurkat and K562 cell using HMOX2 Polyclonal Antibody at dilution of 1:550
Western Blot analysis of Human normal liver tissue and hepg2 cell, Jurkat and K562 cell using HMOX2 Polyclonal Antibody at dilution of 1:550
Western Blot analysis of Human normal liver tissue and hepg2 cell, Jurkat and K562 cell using HMOX2 Polyclonal Antibody at dilution of 1:550

HMOX2 Polyclonal Antibody

E-AB-10367
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetHMOX2
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Overview

  • Supplier
    Elabscience
  • Product Name
    HMOX2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3163
  • Target name
    HMOX2
  • Target description
    heme oxygenase 2
  • Target synonyms
    HO-2, heme oxygenase 2, heme oxygenase (decycling) 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30519
  • Protein Name
    Heme oxygenase 2
  • Scientific Description
    Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Heme oxygenase 2/HMOX2 using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates. Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme oxygenase 2/HMOX2 antigen affinity purified polyclonal antibody (Catalog # A05506-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme oxygenase 2/HMOX2 at approximately 38 kDa. The expected band size for Heme oxygenase 2/HMOX2 is at 36 kDa.
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