HSP40, YDJ1 antibody

Biorbyt

HSP40, YDJ1 antibody

16

0.5 microg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

ImmunoPrecipitation, Western Blot, ELISA

WB (1:2000) ICC, IF, IHC

Research Use Only

Monoclonal

1G10.H8

1 mg/ml

FITC

YDJ1

type I HSP40 co-chaperone YDJ1

HSP40; MAB3; MAS5; type I HSP40 co-chaperone YDJ1; YNL064C

Mouse

IgG1

Mitochondrial protein import protein MAS5

Mouse monoclonal to Hsp40, YDJ1 (FITC). Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. , type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins . The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1..

Yeast

See Manual

12352203