HT-1080 Cell Nuclear Extract

Biorbyt

HT-1080 Cell Nuclear Extract

16

Multi-purpose HT-1080 nuclear extracts are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95° C for 5 minutes. Sample is ready for use in immunoprecipitation and ELISA experiments, conditions should be optimized by the user. Rockland recommends its TrueBlot IP reagents for immunoprecipitation experiments.

ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation

Research Use Only

4 mg/ml

Unconjugated

HT-1080 Cell Nuclear Extract

41105330