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Western Blot analysis of HT-29 cell using HTR3C Polyclonal Antibody at dilution of 1:500
Western Blot analysis of HT-29 cell using HTR3C Polyclonal Antibody at dilution of 1:500
Western Blot analysis of HT-29 cell using HTR3C Polyclonal Antibody at dilution of 1:500

HTR3C Polyclonal Antibody

E-AB-10968
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HTR3C Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit C of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. Genes encoding subunits C, D and E form a cluster on chromosome 3.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HTR3C using anti-HTR3C antibody (A10495-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR3C antigen affinity purified polyclonal antibody (Catalog # A10495-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR3C at approximately 50 kDa. The expected band size for HTR3C is at 50 kDa.
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