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HTRA2 / OMI Antibody

LS-C813172
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHTRA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HTRA2 / OMI Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27429
  • Target name
    HTRA2
  • Target description
    HtrA serine peptidase 2
  • Target synonyms
    epididymis secretory sperm binding protein; high temperature requirement protein A2; HtrA-like serine protease; MGCA8; OMI; Omi stress-regulated endoprotease; PARK13; protease, serine, 25; PRSS25; serine protease 25; serine protease HTRA2, mitochondrial; serine proteinase OMI
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HTRA2 using anti-HTRA2 antibody (A01941-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HT-1080 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA2 antigen affinity purified polyclonal antibody (Catalog # A01941-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA2 at approximately 36 kDa. The expected band size for HTRA2 is at 36,49 kDa.
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