Human ALCAM(Activated Leukocyte Cell Adhesion Molecule) ELISA Kit
ORB2668046
Product group Assays
Overview
- SupplierBiorbyt
- Product NameHuman ALCAM(Activated Leukocyte Cell Adhesion Molecule) ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human ALCAM in the samples is then determined by comparing the OD of the samples to the standard curve
- Assay Detection Range7.81-500 ng/mL
- Assay Sensitivity2.81 ng/mL
- CertificationResearch Use Only
- Scientific DescriptionHuman ALCAM(Activated Leukocyte Cell Adhesion Molecule) ELISA Kit
- UNSPSC41116158