Human Caspase 3 protein, His tag

GeneTex

Human Caspase 3 protein (active)

9

Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. Caspase 3 can hydrolyze the peptide substrate acetyl-Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNA) resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405nm. Thus the activity of recombinant human caspase 3 can be measured by calculate the concentration of the pNA released from the substrate. The reaction was performed in adding 50 microl 2xbuffer (50 mM HEPES, 100 mM NaCl, 10 mM DTT, 2 mM EDTA, 10% glycerol) to 96 well plates, then add 50 microl various concentration of caspe 3 (diluted by 1xbuffer, 25 mM HEPES, 50 mM NaCl, 5 mM DTT, 1 mM EDTA, 5% glycerol) to each well, finally, add 5microl 4mmol Ac-DEVD-pNA to each well. Cover the 96 well plates and incubate at 37C for 2h. p-Nitroaniline (pNA) standard curve prepare by double dilute 200microM pNA with 1xbuffer and record the OD value at 405nm. Calculate the caspase 3 activity in pmol of pNA released per min per microg recombinat human caspase 3. The specific activity of recombinant human caspase 3 is 2196 pmol/min/microg.

Research Use Only

Unconjugated

Lyophilized

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. [provided by RefSeq, Aug 2017]

-20°C or -80°C,2°C to 8°C

12352204