Human Granzyme M protein, His tag (active)

GeneTex

Human Granzyme M protein (active)

9

GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets alpha-Tubulin and disorganizes the microtuble network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2x107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37C for 4h. Samples were immunoblotted using Abs beta-actin as control, and Ezrin to detect the enzyme activity. And the recombinant human GZMM cleaved Ezrin.

Functional Assay

Research Use Only

Unconjugated

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and the protein encoded by this gene, granzyme M. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

-20°C or -80°C,2°C to 8°C

12352204