Fig. 1. Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 was used (analysis in basophil window). Fig. 1A - staining of non-stimulated (control) sample Fig. 1B - staining of IgE stimulated sample