Human IL10 ELISA Kit

Arigo Biolaboratories

Human IL10 ELISA Kit

23

ELISA

1.6 pg/ml

2 h, 2 h, 30 min (RT/shaking)

Research Use Only

Biological activities Human interleukin-10 (IL-10) is a 19 kDA lymphokine produced by T helper lymphocytes,by monocytes, macrophages and B-lymphocytes. IL-10 was first characterized as a cytokine synthesis inhibitory factor (CSIF) able to inhibit cytokine synthesis by TH1 clones activated in the presence of antigen presenting cells. However, in the absence of monocytes, IL-10 directly inhibits the growth of T-cells triggered by immobilized anti-CD3 MoAb. This proliferation inhibition was found to be a result of specific inhibition of IL-2 production by the responding T-cells. In vitro, Il-10 is a very powerful inhibitor of monokines (including TNF-alpha, IL-1, IL-6 and IL-8) produced by LPS-activated monocytes and macrophages. The addition of IL-10 to B lymphocytes results in limited cell proliferation but most importantly in very high immunoglobulin production, a result of the transformation of B-cells into plasma cells. Finally, natural killer (NK) cells appear to be another target for the anti-inflammatory properties of IL-10. Indeed, recent data have shown that IL-10 can inhibit antigen induced IFN-gamma production by NK-cells by inhibiting not only production but also the stimulatory effects of IL-12 and TNF on IFN-gamma production. Clinical application So far, circulating levels of IL-10 have been found in serum of patients suffering of Non-Hodgkins lymphoma, multiple myeloma, cerebral malaria or septic shock.

2°C to 8°C

41116133