Adipogenesis inhibition of 3T3L-1 cells. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1microM, and 10microg/ml, respectively and continued for 2 days (day 0).The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5microg/ml of human Jagged1-Fc (GTX65636-pro) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L-1 cells. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1microM, and 10microg/ml, respectively and continued for 2 days (day 0).The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5microg/ml of human Jagged1-Fc (GTX65636-pro) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Human Jagged 1 protein, human IgG1 Fc tag (active)
GTX65636-PRO
Overview
- SupplierGeneTex
- Product NameHuman Jagged 1 + Human IgG1 Fc fusion protein
- Delivery Days Customer9
- Application Supplier NoteInhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Storage Instruction-20°C
- UNSPSC12352202