Human (mammalian cell expression) recombinant HMGB1 C23AC45AC106A protein, AF

Boster Bio

Human (mammalian cell expression) recombinant HMGB1 C23AC45AC106A protein, AF

9

Other Application

Research Use Only

>98%

High mobility group protein B1

High Mobility Group protein B1 protein (HMGB1) is the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 25 kDa single chain polypeptide containing three domains: two N-terminal HMG boxes A and B, and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu. Post-translational modification on HMGB1 have been reported, affect its localization, receptor interactions, and function. HMGB1, with a disulfide bond between C23 and C45 , have been reported that cause cytokine production and the activation of NF-kappaB. Otherwise, the fully oxidized form has no immune function, losing its proinflammatory effect and the apoptotic cell death activation function. Here, we developed HMGB1 C23A, C45A & 10?A mutant proteins, the fully oxidized HMGB1, eliminant the disulfide bond formation.

-20°C,-20°C or -80°C,2°C to 8°C

12352202