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Human Neopterin ELISA Kit

Research Use Only
ARG80878
Arigo Biolaboratories
Product group Assays
Price on request
Packing Size
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    Human Neopterin ELISA Kit
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Assay Sensitivity
    0.7 nmol/l
  • Assay Time
    90 min (RT/shaker/dark), 10 min (RT)
  • Certification
    Research Use Only
  • Scientific Description
    Neopterin is a low molecular weight molecule belonging to the chemical group known as pteridines. It is synthesised by cellular immune reaction of macrophages and dendritic cells upon stimulation with the cytokine interferon-g and as a consequence released. Neopterin has a higher stability in body fluids which makes the sample handling and measurement easier compared to other cytokines. The low molecular weight, let neopterin molecules rapidly pass the intravasal area, where it is releases in urine after glomerular filtration. The half life period in human bodies is only affected by renal excretion. So neopterin values reflect the totality of immunological processes for monocytes/macrophages and dendritic cells and can be seen as a general marker of immune activity. This characteristic feature of neopterin to reflect the different interactions of immunocompetent cells is the basis for the extraordinary status of measuring neopterin in immmunological diagnosis. As a non-invasive method, urinary neopterin to creatinine ratio determination is also helpful in monitoring disease progression and the effects of therapies, as well. Neopterin biosynthesis is closely associated with activation of the cellular immune system. Increased concentrations of neopterin were reported in patients with viral infections, suggesting that increased values may originate from the immune response of patients directed against virally infected cells. It was shown that antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium and that human macrophages produce neopterin in vitro when stimulated by interferon gamma. The determination of neopterin levels in human body fluids offers a useful and innovative tool to monitor diseases associated with the activation of cell-mediated immunity. Increasing neopterin levels in various infections precede the clinical manifestation and seroconversion. Normally samples are not tested for all possible infections. Therefore, the measurement of neopterin in blood donor samples is a useful tool in order to reduce the risk of infections via blood transfusion.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116158