Human Plasminogen protein, His tag

GeneTex

Human Plasminogen protein (active)

9

Plasminogen (PLG) as a zymogen releases plasmin from the liver into the systemic circulation. Plasmin is a serine protease that acts to dissolve fibrin blood clots. Apart from fibrinolysis, plasmin proteolyses proteins in various other systems: It activates collagenases, some mediators of the complement system and weakens the wall of the Graafian follicle (leading to ovulation). It cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin, like trypsin, belongs to the family of serine proteases. Besides, Insulin Like Growth Factor Binding Protein 3 (IGFBP3) has been identified as an interactor of PLG, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PLG and recombinant human IGFBP3. Briefly, PLG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to IGFBP3-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-PLG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of PLG and IGFBP3 was in a dose dependent manner.

Research Use Only

Unconjugated

Lyophilized

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

-20°C or -80°C,2°C to 8°C

12352204