Human SARS-CoV-2 Nucleocapsid ELISA Kit
ORB867010
ReactivityHuman
Product group Assays
Overview
- SupplierBiorbyt
- Product NameHuman SARS-CoV-2 Nucleocapsid ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve
- ApplicationsELISA
- Applications SupplierELISA
- Assay Detection Range7.82-500 pg/mL
- Assay Sensitivity3.2 pg/mL
- CertificationResearch Use Only
- Scientific DescriptionSandwich Human SARS-CoV-2 Nucleocapsid ELISA Kit
- ReactivityHuman
- UNSPSC41116158