HYAL1 Antibody (aa36-85) LS-B3995

Biorbyt

HYAL1 Antibody (aa36-85) LS-B3995

10

Further information: Immunohistochemistry: LS-B3995 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3995 was determined to be 10 ug/ml. Western Blot: Suggested dilution at 1microg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

IHC, IHC-P (5 µg/ml), WB (1 µg/ml) IHC, IHC-P, WB

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

Rabbit

IgG

HYAL1 Antibody (aa36-85) LS-B3995

Human

-20°C,2°C to 8°C

12352203