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ProductsBack/IARS2 Antibody - N-terminal region (OASG03689)

IARS2 Antibody - N-terminal region (OASG03689)

OASG03689
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetIARS2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    IARS2 Antibody - N-terminal region (OASG03689)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID55699
  • Target name
    IARS2
  • Target description
    isoleucyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    CAGSSS, ILERS, isoleucine--tRNA ligase, mitochondrial, isoleucine tRNA ligase 2, mitochondrial, isoleucine-tRNA synthetase 2, mitochondrial, isoleucyl-tRNA synthetase, mitochondrial, mitochondrial isoleucine tRNA synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IARS2 using anti-IARS2 antibody (A09580-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IARS2 antigen affinity purified polyclonal antibody (Catalog # A09580-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IARS2 at approximately 114 kDa. The expected band size for IARS2 is at 114 kDa.
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