More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Iba1 antibody [GT10312]
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Iba1 antibody [GT10312]

GTX632426
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetAIF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Iba1 antibody [GT10312]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT10312
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor 1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Scientific Description
    This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Huang CC, Chiu HY, Lee PH, et al. Mitochondrial transplantation attenuates traumatic neuropathic pain, neuroinflammation, and apoptosis in rats with nerve root ligation. Mol Pain. 2023,19:17448069231210423. doi: 10.1177/17448069231210423
    Read this paper
  • Kwon H, Lee EH, Park SY, et al. Lactobacillus-derived extracellular vesicles counteract Aβ42-induced abnormal transcriptional changes through the upregulation of MeCP2 and Sirt1 and improve Aβ pathology in Tg-APP/PS1 mice. Exp Mol Med. 2023,55(9):2067-2082. doi: 10.1038/s12276-023-01084-z
    Read this paper
  • Zhou GF, Tang J, Ma YL, et al. ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation. Proc Natl Acad Sci U S A. 2023,120(22):e2220148120. doi: 10.1073/pnas.2220148120
    Read this paper
  • Sun R, Han R, McCornack C, et al. TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma. Sci Adv. 2023,9(19):eade3559. doi: 10.1126/sciadv.ade3559
    Read this paper
  • Yu H, Yu Q, Mi Y, et al. Hydrogen Sulfide Inhibited Sympathetic Activation in D-Galactose-Induced Aging Rats by Upregulating Klotho and Inhibiting Inflammation in the Paraventricular Nucleus. Biomedicines. 2023,11(2). doi: 10.3390/biomedicines11020566
    Read this paper
  • Sakai K, Sugano-Nakamura N, Mihara E, et al. Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions. Nat Biomed Eng. 2023,7(2):164-176. doi: 10.1038/s41551-022-00955-6
    Read this paper
  • Ding Z, Zhong Z, Wang J, et al. Inhibition of Dectin-1 Alleviates Neuroinflammatory Injury by Attenuating NLRP3 Inflammasome-Mediated Pyroptosis After Intracerebral Hemorrhage in Mice: Preliminary Study Results. J Inflamm Res. 2022,15:5917-5933. doi: 10.2147/JIR.S384020
    Read this paper
  • Tang M, Zhao S, Liu JX, et al. Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia. Pharm Biol. 2022,60(1):1556-1565. doi: 10.1080/13880209.2022.2108064
    Read this paper
  • Cao Q, Luo S, Yao W, et al. Suppression of abnormal α-synuclein expression by activation of BDNF transcription ameliorates Parkinson's disease-like pathology. Mol Ther Nucleic Acids. 2022,29:1-15. doi: 10.1016/j.omtn.2022.05.037
    Read this paper
  • Fei X, Dou YN, Wang L, et al. Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage. J Neuroinflammation. 2022,19(1):67. doi: 10.1186/s12974-022-02428-8
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-AIF1 [GT1-mAb1]AB03986-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetAIF1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-AIF1 Antibody144-60080
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAIF1
  • SizePrice
Product group Antibodies
Biosynth
IBA1 antibody70R-21567
  • SizePrice
Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
Product group Antibodies
Boster Bio
Anti-Iba1/AIF1 Antibody Picoband(r)A01394-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetAIF1
  • SizePrice
IHC-P analysis of human placenta tissue using GTX04465 Iba1 antibody [MSVA-955M] HistoMAX?. Early Iba1 expression is particularly strong in macrophages of the placenta.
Product group Antibodies
GeneTex
Iba1 antibody [MSVA-955M] HistoMAX(tm)GTX04465
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetAIF1
  • SizePrice
Iba1 antibody detects Iba1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
References
GeneTex
Iba1 antibodyGTX100042
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAIF1
  • SizePrice
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
References
GeneTex
Iba1 antibodyGTX101495
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAIF1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
Product group Antibodies
References
GeneTex
Iba1 antibody [HL22]GTX635363
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Porcine, Rat
TargetAIF1
  • SizePrice
Iba1 antibody [HL22-MS] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: Iba1 stained by Iba1 antibody [HL22-MS] (GTX635399) diluted at 1:25. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
References
GeneTex
Iba1 antibody [HL22-MS]GTX635399
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAIF1
  • SizePrice
Iba1 antibody [HL22-RT] detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
GeneTex
Iba1 antibody [HL22-RT]GTX635400
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAIF1
  • SizePrice