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Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody

GTX101495
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAIF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Iba1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.14 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor 1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Scientific Description
    This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sun XY, Yu XL, Zhu J, et al. Fc effector of anti-Aβ antibody induces synapse loss and cognitive deficits in Alzheimer's disease-like mouse model. Signal Transduct Target Ther. 2023,8(1):30. doi: 10.1038/s41392-022-01273-8
    Read this paper
  • Li J, Zou B, Cheng XY, et al. Therapeutic effects of total saikosaponins from Radix bupleuri against Alzheimer's disease. Front Pharmacol. 2022,13:940999. doi: 10.3389/fphar.2022.940999
    Read this paper
  • Tsai CM, Chang SF, Li CC, et al. Transcranial photobiomodulation (808 nm) attenuates pentylenetetrazole-induced seizures by suppressing hippocampal neuroinflammation, astrogliosis, and microgliosis in peripubertal rats. Neurophotonics. 2022,9(1):015006. doi: 10.1117/1.NPh.9.1.015006
    Read this paper
  • Jhan MK, Chen CL, Shen TJ, et al. Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus. Cells. 2021,10(11). doi: 10.3390/cells10113181
    Read this paper
  • Navarro-Sempere A, Martínez-Peinado P, Rodrigues AS, et al. The Health Hazards of Volcanoes: First Evidence of Neuroinflammation in the Hippocampus of Mice Exposed to Active Volcanic Surroundings. Mediators Inflamm. 2021,2021:5891095. doi: 10.1155/2021/5891095
    Read this paper
  • Ho MH, Yen CH, Hsieh TH, et al. CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury. Redox Biol. 2021,46:102067. doi: 10.1016/j.redox.2021.102067
    Read this paper
  • Sun XY, Li LJ, Dong QX, et al. Rutin prevents tau pathology and neuroinflammation in a mouse model of Alzheimer's disease. J Neuroinflammation. 2021,18(1):131. doi: 10.1186/s12974-021-02182-3
    Read this paper
  • Lhussiez V, Dubus E, Cesar Q, et al. Cohen Syndrome-Associated Cataract Is Explained by VPS13B Functions in Lens Homeostasis and Is Modified by Additional Genetic Factors. Invest Ophthalmol Vis Sci. 2020,61(11):18. doi: 10.1167/iovs.61.11.18
    Read this paper
  • Baskaran R, Lai CC, Li WY, et al. Characterization of striatal phenotypes in heterozygous Disc1 mutant mice, a model of haploinsufficiency. J Comp Neurol. 2020,528(7):1157-1172. doi: 10.1002/cne.24813
    Read this paper
  • Zhao Z, Fu J, Li S, et al. Neuroprotective Effects of Genistein in a SOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis. J Neuroimmune Pharmacol. 2019,14(4):688-696. doi: 10.1007/s11481-019-09866-x
    Read this paper

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Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
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Iba1 antibody detects Iba1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
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