IgD Fc Antibody (FITC)

LifeSpan BioSciences

IgD Fc Antibody (FITC)

23

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgD molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgD of BALB/C and C57Bl origin used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.. ELISA, ICC, ID, IF, IHC, IHC-Fr The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgD molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgD of BALB/C and C57Bl origin used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.

ImmunoDiffusion, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Research Use Only

Polyclonal

FITC

...

Sheep

-20°C,2°C to 8°C

12352203