Mouse IgG2b Isotype Control FITC

Biorbyt

IgG2b Isotype control (FITC)

16

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 microg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Flow Cytometry

FC

Research Use Only

Monoclonal

MPC-11

1 mg/ml

FITC

IgG2b

Mouse monoclonal antibody conjugated to FITC which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.

2°C to 8°C

12352203