IHC-plus RAMP3 Antibody LS-B15834

Biorbyt

IHC-plus RAMP3 Antibody LS-B15834

10

Further information: Western blot analysis of RAMP3 expression in MCF7 (A), mouse brain (B), mouse heart (C), rat brain (D), rat lung (E) whole cell lysates. Immunofluorescent analysis of RAMP3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) ICC, IF, IHC-P, WB

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

Rabbit

IHC-plus RAMP3 Antibody LS-B15834

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203