IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (aa460-480)

LifeSpan BioSciences

IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (aa460-480)

23

Reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.. ELISA (1:2000 - 1:10000), Flo, ICC, IF (1:100 - 1:1000), IHC, IHC-P (1:100), WB (1:500 - 1:2000) Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

85 mg/ml

Unconjugated

...

Rabbit

Chicken, Human, Mouse, Rat

12352203