IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (aa460-480)
LS-B2844
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (aa460-480)
- Delivery Days Customer23
- Antibody SpecificityReacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
- Application Supplier NoteImmunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.. ELISA (1:2000 - 1:10000), Flo, ICC, IF (1:100 - 1:1000), IHC, IHC-P (1:100), WB (1:500 - 1:2000) Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration85 mg/ml
- ConjugateUnconjugated
- Estimated Purity...
- HostRabbit
- ReactivityChicken, Human, Mouse, Rat
- UNSPSC12352203