Bio-Connect

IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (aa460-480)

LS-B2844
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (aa460-480)
  • Delivery Days Customer
    23
  • Antibody Specificity
    Reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
  • Application Supplier Note
    Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.. ELISA (1:2000 - 1:10000), Flo, ICC, IF (1:100 - 1:1000), IHC, IHC-P (1:100), WB (1:500 - 1:2000) Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    85 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Host
    Rabbit
  • Reactivity
    Chicken, Human, Mouse, Rat
  • UNSPSC
    12352203