IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (phospho-Ser473)

LifeSpan BioSciences

IHC-plus(tm) AKT1 + AKT2 + AKT3 Antibody (phospho-Ser473)

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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species

Immunohistochemistry: LS-B1183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1183 was determined to be 5 ug/ml.. ELISA (1:15000 - 1:60000), IF, IHC, IHC-P (5 µg/ml), WB (1:200 - 1:1000) Immunohistochemistry: LS-B1183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1183 was determined to be 5 ug/ml.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

Rabbit

IgG

Human, Mouse, Rat

2°C to 8°C or -20°C

12352203