IHC-plus(tm) ALCAM / CD166 Antibody (clone 10F1G12)

LifeSpan BioSciences

IHC-plus(tm) ALCAM / CD166 Antibody (clone 10F1G12)

23

Human

Immunohistochemistry: LS-B3883 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3883 was determined to be 1:200.. ELISA (1:10000), IHC, IHC-P (1:200 - 1:400) Immunohistochemistry: LS-B3883 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3883 was determined to be 1:200.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

10F1G12

Unconjugated

...

ALCAM

activated leukocyte cell adhesion molecule

CD166; CD166 antigen; MEMD

Mouse

IgG1

Human

-20°C,2°C to 8°C

12352203