IHC-plus(tm) ANAPC1 / APC1 Antibody (phospho-Ser377)

LifeSpan BioSciences

IHC-plus(tm) ANAPC1 / APC1 Antibody (phospho-Ser377)

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This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity wi

Immunohistochemistry: LS-B68 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B68 was determined to be 10 ug/ml.. ELISA (1:2000 - 1:10000), IHC, IHC-P (10 µg/ml), WB (1:200 - 1:1000) Immunohistochemistry: LS-B68 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B68 was determined to be 10 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

ANAPC1

anaphase promoting complex subunit 1

anaphase-promoting complex 1 (meiotic checkpoint regulator); anaphase-promoting complex subunit 1; APC1; cyclosome subunit 1; MCPR; mitotic checkpoint regulator; testis-specific gene 24 protein; TSG24

Rabbit

IgG

Human

2°C to 8°C or -20°C

12352203