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IHC-plus(tm) CD34 Antibody (clone QBEND-10, Concentrated)

Research Use Only
LS-B5552
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCD34
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CD34 Antibody (clone QBEND-10, Concentrated)
  • Delivery Days Customer
    23
  • Antibody Specificity
    The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed
  • Application Supplier Note
    The antibody can be used for flow cytometry, immunohistochemistry on frozen and paraffin embedded section, immunoprecipitation and Western blotting. Instructions for use Flow cytometry: Use 10 ul antibody (1:100-1:200 dilution) to label 106 cells in 100 ul Immunohistochemistry: This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended dilution: 1:500 - 1:1000 for immunohistochemistry on paraffin sections. Recommended positive control tissue: Tonsil, bone marrow.. Flo, IHC, IHC-Fr, IHC-P (1:800), IP, WB The antibody can be used for flow cytometry, immunohistochemistry on frozen and paraffin embedded section, immunoprecipitation and Western blotting. Instructions for use Flow cytometry: Use 10 ul antibody (1:100-1:200 dilution) to label 106 cells in 100 ul Immunohistochemistry: This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended dilution: 1:500 - 1:1000 for immunohistochemistry on paraffin sections. Recommended positive control tissue: Tonsil, bone marrow.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    QBEND-10
  • Conjugate
    Unconjugated
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203