IHC-plus(tm) CENPF / CENP-F Antibody (aa1759-2093)

LifeSpan BioSciences

IHC-plus(tm) CENPF / CENP-F Antibody (aa1759-2093)

23

Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-ph

Immunohistochemistry: LS-B3157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3157 was determined to be 10 ug/ml. Western Blot: 1-10 ug/ml. Immunoprecipitation: 1-10 ug/ml. Immunohistochemistry: 10 ug/ml with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at pH 6.0.. IHC, IHC-Fr (10 µg/ml), IHC-P (10 µg/ml), IP (1 - 10 µg/ml), WB (1 - 10 µg/ml) Immunohistochemistry: LS-B3157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3157 was determined to be 10 ug/ml. Western Blot: 1-10 ug/ml. Immunoprecipitation: 1-10 ug/ml. Immunohistochemistry: 10 ug/ml with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at pH 6.0.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

...

CENPF

centromere protein F

AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS

Mouse

IgG2a

Human

-80°C

12352203