IHC-plus(tm) FGFR / FGF Receptor Antibody (phospho-Tyr653/Tyr654)

LifeSpan BioSciences

IHC-plus(tm) FGFR / FGF Receptor Antibody (phospho-Tyr653/Tyr654)

23

Immunohistochemistry: LS-B2833 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2833 was determined to be 1:50. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4uC with gentle shaking, overnight.. IHC, IHC-P (1:50), WB (1:1000) Immunohistochemistry: LS-B2833 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2833 was determined to be 1:50. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4uC with gentle shaking, overnight.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

...

Mouse

IgG2b

-20°C,2°C to 8°C

12352203