IHC-plus(tm) HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122)

LifeSpan BioSciences

IHC-plus(tm) HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122)

23

HIF-1 alpha.

Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml.. ICC, IF (8 - 12 µg/ml), IHC, IHC-Fr (1:100 - 1:5000), IHC-P (1.25 µg/ml), IP (1:10 - 1:1000), WB (1:500 - 1:1000) Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

ESEE122

1 mg/ml

Unconjugated

...

HIF1A

hypoxia inducible factor 1 subunit alpha

ARNT interacting protein; basic-helix-loop-helix-PAS protein MOP1; bHLHe78; class E basic helix-loop-helix protein 78; HIF1; HIF-1A; HIF-1alpha; HIF-1-alpha; HIF1-ALPHA; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1; PASD8

Mouse

IgG1

Bovine, Canine, Human, Mouse, Rat

-20°C or -80°C

12352203