IHC-plus(tm) MAPK1 / ERK2 Antibody (clone 4C11C11C4)

LifeSpan BioSciences

IHC-plus(tm) MAPK1 / ERK2 Antibody (clone 4C11C11C4)

23

Ni-NTA purified full length recombinant ERK2 expressed in E. coli strain BL21 (DE3).

Immunohistochemistry: LS-B2159 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2159 was determined to be 5 ug/ml.. ELISA (1:10000), IHC, IHC-Fr (1:500 - 1:2000), IHC-P (5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B2159 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2159 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

4C11C11C4

Unconjugated

MAPK1

mitogen-activated protein kinase 1

ERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; NS13; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2

Mouse

IgG2a

Human

2°C to 8°C or -20°C

12352203