IHC-plus(tm) NPHS1 / Nephrin Antibody (aa1218-1234)

LifeSpan BioSciences

IHC-plus(tm) NPHS1 / Nephrin Antibody (aa1218-1234)

23

Immunohistochemistry: LS-B106 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B106 was determined to be 20 ug/ml.. ELISA (1:1000), IHC, IHC-P (20 µg/ml) Immunohistochemistry: LS-B106 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B106 was determined to be 20 ug/ml.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

NPHS1

NPHS1 adhesion molecule, nephrin

CNF, NPHN, nephrin, nephrin, NPHS1, nephrin, nephrosis 1, congenital, Finnish type (nephrin), renal glomerulus-specific cell adhesion receptor

Rabbit

Human

-20°C,2°C to 8°C

41116161