IHC-plus(tm) PIM3 / PIM-3 Antibody (aa259-288)

LifeSpan BioSciences

IHC-plus(tm) PIM3 / PIM-3 Antibody (aa259-288)

23

Immunohistochemistry: LS-B3461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3461 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples at this time.. IHC, IHC-P (10 µg/ml), WB (1:1000) Immunohistochemistry: LS-B3461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3461 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples at this time.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

PIM3

Pim-3 proto-oncogene, serine/threonine kinase

pim-3; pim-3 oncogene; serine/threonine kinase Pim-3; serine/threonine-protein kinase pim-3

Rabbit

-20°C,2°C to 8°C

12352203