IHC-plus(tm) POU5F1 / OCT4 Antibody (aa100-200)

LifeSpan BioSciences

IHC-plus(tm) POU5F1 / OCT4 Antibody (aa100-200)

23

OCT4 - Embryonic Stem Cell Marker.

Immunohistochemistry: LS-B85 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B85 was determined to be 2.5 ug/ml.. Flo (1:50 - 1:200), ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC, IHC-Fr, IHC-P (2.5 µg/ml), WB (2 µg/ml) Immunohistochemistry: LS-B85 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B85 was determined to be 2.5 ug/ml.

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

POU5F1

POU class 5 homeobox 1

OCT3; Oct-3; OCT4; Oct-4; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; OTF3; OTF-3; OTF4; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein

Rabbit

Human, Mouse, Porcine

-20°C or -80°C

12352203