IHC-plus(tm) PRKCA / PKC-Alpha Antibody (phospho-Thr497)

LifeSpan BioSciences

IHC-plus(tm) PRKCA / PKC-Alpha Antibody (phospho-Thr497)

23

Amino acids 491 to 501 with pTHR497 of human PRKCA

Immunohistochemistry: LS-B861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B861 was determined to be 10 ug/ml.. ELISA (1:1000), IHC, IHC-P (10 µg/ml) Immunohistochemistry: LS-B861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B861 was determined to be 10 ug/ml.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.1 mg/ml

Unconjugated

...

PRKCA

protein kinase C alpha

AAG6; aging-associated gene 6; PKCA; PKC-A; PKCalpha; PKC-alpha; PKCI+/-; PRKACA; protein kinase C alpha type

Rabbit

Human

-20°C,2°C to 8°C

12352203