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ProductsBack/IHC-plus(tm) PRTN3 / Myeloblastin Antibody (aa88-98)

IHC-plus(tm) PRTN3 / Myeloblastin Antibody (aa88-98)

LS-B10600
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPRTN3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PRTN3 / Myeloblastin Antibody (aa88-98)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 27kD band observed in Human Spleen lysates (calculated MW of 27.8kD according to NP_002768.3). Recommended concentration: 1-3 ug/ml.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:8000), WB (0.3 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 27kD band observed in Human Spleen lysates (calculated MW of 27.8kD according to NP_002768.3). Recommended concentration: 1-3 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5657
  • Target name
    PRTN3
  • Target description
    proteinase 3
  • Target synonyms
    ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP-4, NP4, P29, PR-3, PR3, myeloblastin, C-ANCA antigen, Wegener granulomatosis autoantigen, azurophil granule protein 7, leukocyte proteinase 3, neutrophil proteinase 4, serine proteinase, neutrophil, wegener autoantigen
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody (Catalog # A03863-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRTN3 at approximately 32 kDa. The expected band size for PRTN3 is at 28,32 kDa.
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