IHC-P analysis of human breast tissue using GTX12134 IKB alpha antibody [6A920]. Dilution : 5 microg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
![FACS (Intracellular staining) analysis of THP-1 cells using GTX12134 IKB alpha antibody [6A920]. Red : Primary antibody Green : isotype control Shaded histogram : cell only Antibody amount : 0.25microg/10^6 cells](https://www.genetex.com/upload/website/prouct_img/normal/GTX12134/GTX12134_59_FACS_w_23060519_651.webp "FACS (Intracellular staining) analysis of THP-1 cells using GTX12134 IKB alpha antibody [6A920]. Red : Primary antibody Green : isotype control Shaded histogram : cell only Antibody amount : 0.25microg/10^6 cells")
![WB analysis of (A) Daudi and (B) NIH 3T3 cell lysate using GTX12134 IKB alpha antibody [6A920]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX12134/GTX12134_918_WB_w_23060519_255.webp "WB analysis of (A) Daudi and (B) NIH 3T3 cell lysate using GTX12134 IKB alpha antibody [6A920]. Dilution : 2 microg/ml")
![WB analysis of 100 microg/ml ALLN* (N-Acetyl-Leu-Leu-Norleucinal) treated Jurkat cell followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3) using GTX12134 IKB alpha antibody [6A920] and IKB alpha phospho-specific antibody. *ALLN (N-Acetyl-Leu-Leu-Norleucinal) : Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation](https://www.genetex.com/upload/website/prouct_img/normal/GTX12134/GTX12134_919_WB_w_23060519_948.webp "WB analysis of 100 microg/ml ALLN* (N-Acetyl-Leu-Leu-Norleucinal) treated Jurkat cell followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3) using GTX12134 IKB alpha antibody [6A920] and IKB alpha phospho-specific antibody. *ALLN (N-Acetyl-Leu-Leu-Norleucinal) : Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation")
![WB analysis of Jurkat, LNCaP, PC-3, HeLa, NIH3T3 cell lysates using GTX12134 IKB alpha antibody [6A920]. Dilution : 0.5 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX12134/GTX12134_920_WB_w_23060519_583.webp "WB analysis of Jurkat, LNCaP, PC-3, HeLa, NIH3T3 cell lysates using GTX12134 IKB alpha antibody [6A920]. Dilution : 0.5 microg/ml")
![WB analysis of NIH-3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].](https://www.genetex.com/upload/website/prouct_img/normal/GTX12134/GTX12134_921_WB_w_23060519_210.webp "WB analysis of NIH-3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].")
IHC-P analysis of human breast tissue using GTX12134 IKB alpha antibody [6A920]. Dilution : 5 microg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
IKB alpha antibody [6A920]
GTX12134
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNFKBIA
Overview
- SupplierGeneTex
- Product NameIKB alpha antibody [6A920] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1 - 2 microg/ml. ICC/IF: 1:20 - 1:1000. IHC-P: 1:100. FACS: 0.25 - 1 microg / 106 cells. IP: 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6A920
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4792
- Target nameNFKBIA
- Target descriptionNFKB inhibitor alpha
- Target synonymsEDAID2; IkappaBalpha; I-kappa-B-alpha; IKBA; ikB-alpha; MAD-3; major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
- HostMouse
- IsotypeIgG1
- Protein IDP25963
- Protein NameNF-kappa-B inhibitor alpha
- Scientific DescriptionNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203