Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-1 Alpha Polyclonal Antibody, Unconjugated (bs-4946R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-1 Alpha Polyclonal Antibody, Unconjugated (bs-4946R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
IL-1 Alpha Polyclonal Antibody
BS-4946R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIL1A
Overview
- SupplierBioss Antibodies
- Product NameIL-1 Alpha Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3552
- Target nameIL1A
- Target descriptioninterleukin 1 alpha
- Target synonymshematopoietin-1; IL1; IL-1 alpha; IL-1A; IL1-ALPHA; IL1F1; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
- HostRabbit
- IsotypeIgG
- Protein IDP01583
- Protein NameInterleukin-1 alpha
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203