IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.)

AdipoGen Life Sciences

IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.)

10

Research Use Only

Interleukin-12 (IL-12) family members are heterodimer glycoproteins, composed of two covalently linked subunits, alpha and beta chains. The alpha-subunit consists of IL-23p19, IL-27p28, and IL-12p35 and the beta-subunit includes IL-12p40 and Epstein-Barr virus-induced gene (Ebi3). IL-12 members bind to cognate heterodimeric receptor chains expressed on T cells. This family includes IL-12, IL-23, IL-27 and IL-35 and IL-39. IL-12 and IL-23 are predominantly proinflammatory cytokines that contribute key roles in the development of Th1 and Th17 cells, respectively. IL-27 has both pro- and anti-inflammatory properties and is a potent T cell immunomodulator. IL-35, a new member of this family, is a potent inhibitory cytokine produced by natural, thymus-derived regulatory T cell (nTreg) populations. Recently, a subset of B cells that produce IL-35 was identified and shown to provide protection against autoimmune diseases. These IL-12 family members link innate immunity with the development of adaptive immunity and are also important for regulating T cell responses. IL-35 is composed of two subunits, IL-12A and Epstein-Barr virus-induced gene 3 (EBI3) signals via a heterodimeric receptor consisting of IL-12Rbeta2 and glycoprotein (gp130), leading to activation of STAT 1 and 4. IL-35 is a potent suppressive cytokine directly limiting the inflammatory response that has important roles in infection, cancer and autoimmune diseases. The protein IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-12A:Fc Knobs sequence (synthesizing a protein of 68kDa) and one encoding for the IL27B/EBI3:Fc Holes sequence (synthesizing a protein of 60kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences. - InVivoKines™. Recombinant Protein. IL-12Ap35 (aa 23-219) (human):Fc (LALA-PG) Knobs and IL-27B/EBI3 (aa 21-229) (human):Fc (LALA-PG) Holes form the IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). Source: HEK 293 cells. Produced using animal component-free medium.. Lyophilized. Contains PBS Interleukin-12 (IL-12) family members are heterodimer glycoproteins, composed of two covalently linked subunits, alpha and beta chains. The alpha-subunit consists of IL-23p19, IL-27p28, and IL-12p35 and the beta-subunit includes IL-12p40 and Epstein-Barr virus-induced gene (Ebi3). IL-12 members bind to cognate heterodimeric receptor chains expressed on T cells. This family includes IL-12, IL-23, IL-27 and IL-35 and IL-39. IL-12 and IL-23 are predominantly proinflammatory cytokines that contribute key roles in the development of Th1 and Th17 cells, respectively. IL-27 has both pro- and anti-inflammatory properties and is a potent T cell immunomodulator. IL-35, a new member of this family, is a potent inhibitory cytokine produced by natural, thymus-derived regulatory T cell (nTreg) populations. Recently, a subset of B cells that produce IL-35 was identified and shown to provide protection against autoimmune diseases. These IL-12 family members link innate immunity with the development of adaptive immunity and are also important for regulating T cell responses. IL-35 is composed of two subunits, IL-12A and Epstein-Barr virus-induced gene 3 (EBI3) signals via a heterodimeric receptor consisting of IL-12Rbeta2 and glycoprotein (gp130), leading to activation of STAT 1 and 4. IL-35 is a potent suppressive cytokine directly limiting the inflammatory response that has important roles in infection, cancer and autoimmune diseases. The protein IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-12A:Fc Knobs sequence (synthesizing a protein of 68kDa) and one encoding for the IL27B/EBI3:Fc Holes sequence (synthesizing a protein of 60kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

-20°C,2°C to 8°C

12352204