IL1 alpha antibody [364-3B3-14]

GeneTex

IL1 alpha antibody [364-3B3-14]

9

ELISA: Use at a concentration of 2 - 6 microg/ml. To obtain a linear standard curve, serial dilutions of IL-1 alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. ELISPOT: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. This antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1 alpha -producing cells within mixed cell populations. Optimal dilutions/concentrations should be determined by the end user.

ELISA

Research Use Only

Monoclonal

364-3B3-14

0.5 mg/ml

Unconjugated

Mouse

IgG1

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Human

-20°C or -80°C,2°C to 8°C

12352203