IL12 Antibody

LifeSpan BioSciences

IL12 Antibody

23

This antibody recognizes both the p35 and p40 sub-units of canine IL-12. Cross-reactivity (direct ELISA): ~40% with recombinant human IL-12 p35/p40 and recombinant feline IL-12 p35/p40; <10% with recombinant rat IL-12 p35/p40.

Suitable for use in ELISA, Western Blot and Neutralization. ELISA (capture): 0.2-0.8 ug/ml. Western Blot: 0.1-0.2 ug/ml. Detection limit for rcaIL-12 is ~50 ng/lane under non-reducing and reducing conditions. Neutralization: The ND 50 was determined to be ~0.1-0.5 ug/ml in the presence of 25 ng/ml of rcaIL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs).. ELISA, Neut, WB (0.1 - 0.2 µg/ml) Suitable for use in ELISA, Western Blot and Neutralization. ELISA (capture): 0.2-0.8 ug/ml. Western Blot: 0.1-0.2 ug/ml. Detection limit for rcaIL-12 is ~50 ng/lane under non-reducing and reducing conditions. Neutralization: The ND 50 was determined to be ~0.1-0.5 ug/ml in the presence of 25 ng/ml of rcaIL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Polyclonal

Unconjugated

...

Goat

IgG

Canine

-20°C

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