IL12B: IL12RB1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

BPS Bioscience

IL12B: IL12RB1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

7

Neutralisation/Blocking

Research Use Only

Biotin

Interleukin-12 receptor subunit beta-1

The IL12B: IL12RB1[Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL12B (Interleukin 12B) and IL12RB1 (Interleukin 12 receptor subunit beta 1) for screening and profiling applications. This kit comes with enough purified IL12B (amino acids 23-328(end)) and biotinylated IL12RB1(amino acids 24-540), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL12B: IL12RB1[Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL12B protein. After coating and blocking, biotinylated IL12RB1 is added in an optimized assay buffer. Next, unbound biotinylated IL12RB1 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL12RB1 binding to IL12B. Need us to run inhibitor screens or profile your compounds against IL12B binding to IL12RB1? Check out our Immunotherapy Screening Services.

41116100

Human