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ProductsBack/IL17F Antibody (Biotin)

IL17F Antibody (Biotin)

LS-C154144
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL17F
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL17F Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.9 kD in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA (1:20000 - 1:100000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000) This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.9 kD in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID112744
  • Target name
    IL17F
  • Target description
    interleukin 17F
  • Target synonyms
    CANDF6; cytokine ML-1; IL-17F; interleukin-17F; ML1; ML-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17F using anti-IL17F antibody (A02062-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17F antigen affinity purified polyclonal antibody (Catalog # A02062-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17F at approximately 18,30KD. The expected band size for IL17F is at 18KD.
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