iLite® Type I IFN Assay Ready Cells are type I IFN responsive reporter gene cells designed for use in assays measuring the functional activity of type I Interferon (IFN) or the presence of neutralizing antibodies (NAbs) against type I IFN.
Due to the functional nature of the assay, both Type I IFN, and any inhibitory agents against Type I IFN or its receptor, can be measured using the same assay.
The iLite Type I IFN cells are human cells (U937) engineered to express Firefly Luciferase under the control of an IFN α/β responsive promoter. When IFNα or IFNβ binds to the IFN α/β receptor on the cell surface, the IFN α/β regulated Firefly Luciferase reporter gene construct will be activated, resulting in a luminescent signal.
The cells can be used for measuring the levels of Type I Interferon (38 samples) or neutralizing antibodies to Type I Interferon (Interferon beta or Interferon alpha) (20 samples).
