IMMUNIS HBV Genotype EIA

Cosmo Bio USA

IMMUNIS HBV Genotype EIA

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This product is an enzyme immunoassay (EIA) based HBV genotyping kit, designed to determine genotype (A, B, C, or D) by detecting genotype-specific epitopes in PreS2 region . Many studies indicate that HBV genotypes influence the severityof chronic hepatitis B and the response to interferon therapy. HBV genotypes have been determined by tedious methods such as direct sequencing, genotype-specific PCR, PCR-RFLP, line-probe assay (LiPA), or PCR-invader assay. Background There are eight genotypes (genotype A to H) of hepatitis B virus (HBV) which are distinguished by > 8 % difference in the nucleotide sequence of the HBV genome. Genotypes A and D are common in Europe and the United States, while genotypes B and C are prevalent in Asia. Genotype F is frequent in Latin America, while genotype E is confined to Central and Western Africa. Many studies indicate that HBV genotypes influence the severity of chronic hepatitis B and the response to interferon therapy. HBV genotypes have been determined by tedious methods such as direct sequencing, genotype-specific PCR, PCR-RFLP, line-probe assay (LiPA), or PCR-invader assay. This product is an enzyme immunoassay (EIA) based HBV genotyping kit, designed to determine genotype (A, B, C, or D) by detecting genotype-specific epitopes in PreS2 region . Feature and Advantages The first diagnostic reagent for determination of HBV genotype launched in the Japanese market Fast and easy detection based on one-step EIA method 98% of samples with HBsAg concentration of >3 IU/mL can be genotyped (unpublished data) Sample volume: 80microL of HBsAg positive serum Stored samples can be used Application sample Hepatitis B Virus serological genotyping in human serum (Genotype A, B, C, or D) Determination of HBV genotypes Determine genotypes by the positive/negative combinations (serotypes) of four epitopes (m, k, s, or u) in accordance with the table below.Sample sera showing serotypes other than su, m, ks, or ksu can not be classified by this kit. Kit Contents References Okamoto H, Tsuda F, Sakugawa H, et al:Typing hepatitis B virus by homology in nucleotide sequence:comparison of surface antigen subtypes. J Gen Virol 69:2575-2583, 1988. Arauz-Ruiz P, Norder H, Robertson BH, et al:Genotype H:a new Amerindian genotype of hepatitis B virus revealed in Central America. J Gen Virol 83:2059-2073, 2002. Orito E, Ichida T, Sakugawa H, et al:Geographic distribution of hepatitis B virus (HBV) genotype in patients with chronic HBV infection in Japan. Hepatology 34:590-594, 2001. Usuda S, Okamoto H, et al: Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product. J Virol Methods 80 : 97-112, 1999. ,,,: HBV EIA. 57: 42-47, 2009. Kobayashi M, Suzuki F, Arase Y, et al:Infection with hepatitis B virus genotype A in Tokyo, Japan during 1976 through 2001. J Gastroenterol 39:844-850, 2004. Suzuki Y, Kobayashi M, Ikeda K, et al:Persistence of acute infection with hepatitis B virus genotype A and treatment in Japan. J Med Virol 76:33-39, 2005. Ozasa A, Tanaka Y, Orito E, et al:Influence of genotypes and precore mutations on Fulminant or Chronic outcome of acute hepatitis B virus infection. Hepatology 44:326-334,2006. Kao JH, Wu NH, Chen PJ, et al:Hepatitis B genotypes and the response to interferon therapy. J Hepatol 33:998-1002, 2000. Wai CT, Chu CJ, Hussain M, et al:HBV genotype B is associated with better response to interferon therapy in HBeAg(+) chronic hepatitis than genotype C. Hepatology 36 : 1425-1430, 2002 McMahon BJ:The influence of hepatitis B virus genotype and subgenotype on the natural history of chronic hepatitis B. Hepatol Int 3:334-342, 2009

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