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IP10 Antibody (YA2542)

HY-P82797
MedChem Express
TargetCXCL10
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    IP10 Antibody (YA2542)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3627
  • Target name
    CXCL10
  • Target description
    C-X-C motif chemokine ligand 10
  • Target synonyms
    10 kDa interferon gamma-induced protein; C7; crg-2; C-X-C motif chemokine 10; gamma IP10; gIP-10; IFI10; INP10; interferon-inducible cytokine IP-10; IP-10; mob-1; protein 10 from interferon (gamma)-induced cell line; SCYB10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    IP10 Antibody (YA2542) is a non-conjugated IgG antibody, targeting IP10, with a predicted molecular weight of 11 kDa (observed band size: 11 kDa). IP10 Antibody (YA2542) can be used for WB experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IP10 using anti-IP10 antibody (PB9695). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP10 antigen affinity purified polyclonal antibody (Catalog # PB9695) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP10 at approximately 34 kDa. The expected band size for IP10 is at 11 kDa.
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